Antigen

Antigen Class

TFs and others

Antigen

KMT2A

Cell type

Cell type Class

Blood

Cell type

Acute lymphoid leukemia

NA

NA

Sample

source_name

MLL-AF4 infant ALL patient 2 blasts

disease state

acute lymphoblastic leukemia (ALL)

treatment

N/A

individual

1-year old MLL-AF4 ALL patient

tissue

Bone marrow aspirate

chip antibody

Bethyl A300-086A

geo_loc_name

missing

collection_date

missing

Sequenced DNA Library

library_name

GSM6122657

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were washed with PBS and fixed in 2mM DSG for 30 minutes and 1% FA for 30 minutes. Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext Ultra DNA II library preparation kit for Illumina (Cat no. E7645).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

59292895

Reads aligned (%)

98.0

Duplicates removed (%)

4.0

Number of peaks

39678 (qval < 1E-05)

hg19

Number of total reads

59292895

Reads aligned (%)

97.3

Duplicates removed (%)

4.1

Number of peaks

39297 (qval < 1E-05)