Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KMT2A

Cell type

Cell type Class
Blood
Cell type
Acute lymphoid leukemia
NA
NA

Attributes by original data submitter

Sample

source_name
MLL-AF4 infant ALL patient 2 blasts
disease state
acute lymphoblastic leukemia (ALL)
treatment
N/A
individual
1-year old MLL-AF4 ALL patient
tissue
Bone marrow aspirate
chip antibody
Bethyl A300-086A
geo_loc_name
missing
collection_date
missing

Sequenced DNA Library

library_name
GSM6122657
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were washed with PBS and fixed in 2mM DSG for 30 minutes and 1% FA for 30 minutes. Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext Ultra DNA II library preparation kit for Illumina (Cat no. E7645).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
59292895
Reads aligned (%)
98.0
Duplicates removed (%)
4.0
Number of peaks
39678 (qval < 1E-05)

hg19

Number of total reads
59292895
Reads aligned (%)
97.3
Duplicates removed (%)
4.1
Number of peaks
39297 (qval < 1E-05)

Base call quality data from DBCLS SRA